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<p><b>OBJECTIVE</b>This review aims to systematically evaluate the effect of decalcified freeze-dried bone allograft (DFDBA) combined with rich platelet derivatives on the treatment of human periodontal intrabony defects.</p><p><b>METHODS</b>A search in PubMed, Web of Science, Embase, Cochrane Library, CNKI, and other electronic databases was conducted to identify randomized controlled trials (RCT) of the use of DFDBA combined with rich platelet derivatives in the treatment of human periodontal intrabony defects, performed before May 2016. The quality of the RCTs was assessed. RevMan 5.3 software was applied for Meta-analysis.</p><p><b>RESULTS</b>A total of nine RCTs were included. A total of 194 patients and 303 defects were involved. Short-term (6 months) and long-term (12 to 18 months) groups were included. Meta-analysis results revealed that DFDBA combined with rich platelet derivatives was superior to DFDBA or rich platelet derivatives alone for probing depth reduction in the short-term [MD=0.75 mm, 95% confidence intervals (CI) (0.31 mm, 1.20 mm), P=0.001 0] and longterm groups [MD=0.87 mm, 95%CI (0.02 mm, 1.72 mm), P=0.04], clinical attachment level gain in the short-term [MD= 0.65 mm, 95%CI (0.08 mm, 1.22 mm), P=0.03] and long-term groups [MD=1.31 mm, 95%CI (0.60 mm, 2.01 mm), P<0.000 3], gingival recession reduction in the long-term group [MD=-0.58 mm, 95%CI (-0.78 mm, -0.38mm), P<0.000 01], bone fill gain in the short-term [MD=0.52 mm, 95%CI (0.03 mm, 1.00 mm), P=0.04] and long-term groups [MD=1.26 mm, 95%CI (0.65 mm, 1.86 mm), P<0.000 1].</p><p><b>CONCLUSIONS</b>DFDBA combined with platelet rich derivatives is probably effective in the treatment of human periodontal intrabony defects. It is probably superior to DFDBA or platelet rich derivatives alone. Considering the limitation of the included studies, high-quality and large-sample RCTs are required to evaluate the effect.</p>
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The drug metabolism is a biochemical process on modification of pharmaceutical substances through specialized enzymatic systems. Changes in the expression of drug-metabolizing enzyme genes can affect drug metabolism. Recently, epigenetic regulation of drug-metabolizing enzyme genes has emerged as an important mechanism. Epigenetic regulation refers to heritable factors of genomic modifications that do not involve changes in DNA sequence. Examples of such modifications include DNA methylation, histone modifications, and non-coding RNAs. This review examines the widespread effect of epigenetic regulations on genes involved in drug metabolism, and also suggests a network perspective of epigenetic regulation. The epigenetic mechanisms have important clinical implications and may provide insights into effective drug development and improve safety of drug therapy.
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Objective To establish an in-vitro root canal-apical complex model for studying the mechanisms of the infected root canal to apical periodontitis and periapical biofilm .Methods Single rooted premolar extracted for orthodontic ,was sealed in sterile vial containing LB solid medium ,and the culture medium covered apical thirds .Totally 25 root canal-apical complex models were prepared .Five models were randomly selected for the bacteria detection in periapical by PCR at 1st day .The remained 20 models were randomly subjected to a control group(n=10) and experimental group(n=10) .Extracted teeth were opened in experimental group and control group with no treatment .All models were exposed in air .At 21st day ,bacteria were detected through PCR in root canal and apical;endotoxin content in apical was assayed by chromogenic end-point limulus test .Results In apical ,bacteria was not found in all groups ,but not for the experimental group .The mean endotoxin content was (8 .913 ± 0 .614)EU/mL in control group and (10 .525 ± 0 .981)EU/mL in experimental group .The endotoxin content was increased significantly in experimental group ,when compared with control group(P<0 .01) .Conclusion Root canal-apical complex was established in vitro through this method .Bac-teria was not easy to reach the apical when the infected root canal was not disturbed .Bacteria in the infected root canal caused apical periodontitis through the secretion of virulence factors such as endotoxin .
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The root canal system of mandibular second molar is complex.This article presents one case with type 1-2 mesial root canal of mandibular second molar.The canal was treated by warm gutta percha,and conformed by X-ray examination and dental operating microscopy.
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Objective To investigate the effect of Pudilan combined with ribavirin in treatment of the hand-foot-mouth disease(HFMD). Methods 70 patients were randomly divided into treatment group ( Pudilan combined with ribavirin) and control group (ribavirin only). Results Compared with the control group,the cure rate and the effective rate in treatment group had statistically significant difference ( all P ＜ 0.05 ). Compared with the control group,the rash disappeared time and the fever subsiding time in treatment group were shorter obviously ( all P ＜0. 05 ). The both groups had no obvious side effect. Conclusion Pudilan combined with ribavirin in the treatment of HFMD had a good therapeutic effect and were a safe and effective method.
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Objective To investigate whether ultrasound-mediated microbubble destruction can effectively deliver pEGFP-N1 plasmid into human gingival fibroblasts (HGFs).Methods The primary cultured HGFs were divided into 4 groups,that is,plasmid,microbubble+plasmid,ultrasound+plasmid,and ultrasound+microbubble+plasmid groups.pEGFP-N1 plasmid was used to transfect to HGFs as a gene marker with ultrasound-mediated microbubble destruction.The last group was further divided into subgroups to optimize the transfection conditions.After 48 h,phase-contrast fluorescent microscopy was employed to evaluate the expression of green fluorescent protein (GFP).The cell vitality was measured by the MTT assay.Results The transfection efficiency of the ultrasound+microbubble+plasmid group was higher than other experiment groups.Optimal gene expression was found when ultrasound was radiated at 1.5 W/cm2 for 60 s,microbubble was at a concentration of 10% and plasmid was at a concentration of 6.67 ?g/ml,and the transfection efficiency was highest under this condition.Conclusion Under specific conditions,ultrasound mediated microbubble destruction enhances the reporter gene transfection and expression in HGFs.
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OBJECTIVE:To study the effects and security of amlexanox oral coating for treatment of minor recurrent aphthous ulcer(MIAU). METHODS:A randomized double-blind experiment was carried on 42 patients with MIAU were randomly divided into experimental group(20 patients),and control group(22 patients),each group respectively administered with amlexanox coating and placebo,at the lesion site 4 times a day for 5 days.RESULTS:At 3 days,the effective rates of healing in the experimental and control group were 85.0% and 36.4%,respectively(P